منابع مشابه
CD34 enrichment of umbilical cord blood.
OBJECTIVE We describe the relation between the clamping time and blood volume collected, plus two enrichment systems for CD34+ stem cells from umbilical cord blood, to achieve an excellent recovery of cells with a high proliferative ability and bone marrow reconstitution. METHOD After cord blood collection by an obstetrician, stem cell purification has been performed either with a combination...
متن کاملCD34+ stem cells from umbilical cord blood
We describe the relation between umbilical cord clamping time and two different enrichment system of CD34(+) stem cells from umbilical cord blood with the proliferative ability and bone marrow reconstitution of the stem cells obtained. After an obstetrician performed the cord blood collection, the purification of stem cells was performed either with a combination of monoclonal antibodies (negat...
متن کاملCorrelation of umbilical cord blood volume with CD34+ cells concentration
Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematopoitic progenitor cells for clinical application. The parameters commonly used to evaluate a UCB unit and predict transplant outcomes have been total nucleated cell count (TNCs), CD34+ cells concentration and total volume of cord blood collected. The volume of cord blood collection is also important ...
متن کاملEnrichment, characterization, and responsiveness of single primitive CD34 human umbilical cord blood hematopoietic progenitors with high proliferative and replating potential.
To characterize the growth of cord blood progenitor cells, single nonadherent, low-density, T-lymphocyte-depleted CD34 cells were sorted by flow cytometer with an autoclone device into single wells containing culture medium and cytokines. These cells were evaluated for proliferation and for replating ability of their progeny. This latter effect is used as a measure of self-renewal capacity. Col...
متن کاملUmbilical cord blood transplantation
Since the first umbilical cord blood transplantation (CBT) in 1998, cord blood (CB) has now become one of the most commonly used sources of hematopoietic stem cells for transplantation. CBT has advantages of easy procurement, no risk to donor, low risk of transmitting infections, immediate availability and immune tolerance allowing successful transplantation despite human leukocyte antigen disp...
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ژورنال
عنوان ژورنال: Bratislava Medical Journal
سال: 2013
ISSN: 1336-0345
DOI: 10.4149/bll_2013_081